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Student: Expression of Salmonella typhimurium virulence factors during the infection
| Group: | Hardt |
| Position: | Semesterarbeit / Diplomarbeit |
| Contact: |
Prof. Dr. Wolf Dietrich Hardt Institute of Microbiology Wolfgang-Pauli-Str. 10 HCI G 417 ETH Zürich CH-8093 Zürich Phone: +41(0)44 632 5143 E-mail: hardt@micro.biol.ethz.ch |
| Description: |
Salmonella typhimurium is one of the leading causes of bacterial food poisoning. The infection requires a large array of different virulence factors (Toxins, components of the Type III secretion apparatus etc.). The expression of these virulence factors must be tightly regulated in order to ensure that the right set of virulence factors is expressed at the appropriate stage of the disease. The project is going to analyze the expression of several different virulence factors, including the SPI-1 Type III secretion system and several key SPI-1 effector proteins (i.e. SopE (Buchwald et al., 2002; Hardt et al., 1998)). To measure gene expression the student will use reporter gene techniques (GFP and luciferase), Western Blot, Immunofluorescence microscopy as well as real time RT-PCR. Gene expression will be analyzed in vitro, using tissue culture infection models and (in collaboration with a PhD student) in a new animal model (Barthel et al., 2003). Literature:
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as agreed |
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