Publications of the Vorholt Lab since 1995

• PubMed Listing  »»

2012

• Stiefel P, Schmidt FI, Dörig P, Behr P, Zambelli T, Vorholt JA, Mercer J (2012) Cooperative vaccinia infection demonstrated at the single-cell level using FluidFM. Nano. Lett. 12(8):4219-27
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• Vogel C, Innerebner G, Zingg J, Guder J, Vorholt JA (2012) A forward genetic in planta screen for the identification of plant-protective traits of Sphingomonas sp. Fr1 against Pseudomonas syringae DC3000. Appl. Environ. Microbiol. ...
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• Campagne S, Damberger FF, Kaczmarczyk A, Francez-Charlot A, Allain FH-T, Vorholt JA (2012) Structural basis for sigma factor mimicry in the general stress response of Alphaproteobacteria. Proc. Natl. Acad. Sci. USA. 109:E1405-14.
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• Schneider K, Skovran E, Vorholt JA (2012) Oxalyl-CoA reduction to glyoxylate ist he preferred route of oxalate assimilation in Methylobacterium extorquens AM1. J. Bacteriol. 194:3144-3155.
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• Kaczmarczyk A, Vorholt JA, Francez-Charlot A (2012) A markerless gene deletion system for Sphingomonads. Appl. Environ. Microbiol. 78:3774-3777.
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• Atamna-Ismaeel N, Finkel O, Glaser F, von Mering C, Vorholt JA, Koblizek M, Belkin S and Béjà O (2012) Bacterial aerobic anoxygenic photosynthesis on plant leaf surfaces. Environ. Microbiol. Rep. 4:209-216.
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• Schneider K, Peyraud R, Kiefer P, Delmotte N, Portais J.-C., Vorholt JA (2012) The ethylmalonyl-CoA pathway is used in place of the glyoxylate cycle by Methylobacterium extorquens AM1 during growth on acetate. J. Biol. Chem. 287:757-766.
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• Knief C, Dengler V, Bodelier PLE, Vorholt JA (2012) Characterization of Methylobacterium strains isolated from the phyllosphere and description of new species. Antonie v Leuwenhoek. 101:169-83.
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• Atamna-Ismaeel N, Finkel OM, Glaser F, Sharon I, Schneider R, Post AF, Spudich JL, von Mering C, Vorholt JA, Iluz D, Béjà O and Belkin S (2012) Bacterial rhodopsins on leaf surfaces of terrestrial plants. Environm. Microbiol. 14:140-146.
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2011

• Peyraud P, Schneider K, Kiefer P, Massou S, Vorholt JA, Portais J-C (2011) Genome-scale reconstruction and system level investigation of the metabolic network of Methylobacterium extorquens AM1. BMC Systems Biol. 5:189.
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• Kaczmarczyk A, Danza F, Campagne S, Metzger L, Vorholt JA, Francez-Charlot A (2011) The PhyR-NepR-SigmaEcfG cascade in Sphingomonas sp. FR1: role in general stress response and identification of a negative regulator of PhyR. J. Bacteriol. 193:6629-38
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• Kuntze K, Kiefer P, Baumann S, von Bergen M, Vorholt JA, Boll M (2011) Enzymes involved in the anaerobic degradation of meta-substituted halobenzoates. Mol. Microbiol. 82:758-769.
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• Gourion B, Delmotte N, Bonaldi K, Nouwen N, Vorholt JA, Giraud E (2011) Bacterial RuBisCO is required for efficient Bradyrhizobium/Aeschynomene symbiosis. PLos ONE 6:321900.
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• Knief C, Delmotte N, Vorholt JA (2011) Bacterial adaptation to life in association with plants - A proteomic perspective from culture to in situ conditions. Proteomics. 11(15):3086-105.
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• Innerebner G, Knief C, Vorholt JA (2011) Protection of Arabidopsis thaliana against leaf-pathogenic Pseudomonas syringae by Sphingomonas strains in a controlled model system. Appl Environ Microbiol. 77(10):3202-10.
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• Kiefer P, Delmotte N, Vorholt JA (2011) Nanoscale ion-pair reversed-phase HPLC-MS for sensitive metabolome analysis. Anal Chem. 83(3):850-5 (2011).
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2010

• Knief C, Frances L, Vorholt JA (2010) Competitiveness of Diverse Methylobacterium Strains in the Phyllosphere of Arabidopsis thaliana and Identification of Representative Models, Including M. extorquens PA1. Microb Ecol. 60(2):440-52.
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• Koch M, Delmotte N, Rehrauer H, Vorholt JA, Pessi G, Hennecke H (2010) Rhizobial adaptation to hosts, a new facet in the legume root-nodule symbiosis. Mol Plant Microbe Interact. 23(6):784-90.
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• Dörig P, Stiefel P, Behr P, Sarajlic E, Bijl D, Gabi M, Vörös J, Vorholt JA, Zambelli T (2010) Force-controlled spatial manipulation of viable mammalian cells and micro-organisms by means of FluidFM technology. Appl. Phys. Lett. 97, 023701 (2010).
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• Schmidt S, Christen P, Kiefer P, Vorholt JA (2010) Functional investigation of methanol dehydrogenase-like protein XoxF in Methylobacterium extorquens AM1. Microbiology, 156(Pt 8):2575-86.
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• Knief C, Ramette A, Frances L, Alonso-Blanco C, Vorholt JA (2010) Site and plant species are important determinants of the Methylobacterium community composition in the plant phyllosphere. ISME J. 10.1038/ismej.2010.9.
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• Delmotte N, Ahrens CH, Knief C, Qeli E, Koch M, Fischer HM, Vorholt JA, Hennecke H, Pessi G (2010) An integrated proteomics and transcriptomics reference data set provides new insights into the Bradyrhizobium japonicum bacteroid metabolism in soybean root nodules. Proteomics 10(7):1391-400.
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2009

• Takeuchi K, Kiefer P, Reimmann C, Keel C, Dubuis C, Rolli J, Vorholt JA, Haas D (2009) Small RNA-dependent expression of secondary metabolism is controlled by Krebs cycle function in Pseudomonas fluorescens. J. Biol. Chem. 11;284(50):34976-85.
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• Kiefer P, Buchhaupt M, Christen P, Kaup B, Schrader J, Vorholt JA (2009) Metabolite profiling uncovers plasmid-induced cobalt limitation under methylotrophic growth conditions. PLoS One 13;4(11):e7831.
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• Delmotte N, Knief C, Chaffron S, Innerebner G, Roschitzki B, Schlapbach R, von Mering C, Vorholt JA (2009) Community proteogenomics reveals insights into the physiology of phyllosphere bacteria. Proc. Natl. Acad. Sci USA 106(38):16428-16433.
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• Gourion B, Sulser S, Frunzke J, Francez-Charlot A, Stiefel P, Pessi G, Vorholt JA, Fischer HM (2009) The PhyR-s^EcfG signaling cascade is involved in stress response and symbiotic efficiency in Bradyrhizobium japonicum. Mol. Microbiol. 73:291-305.
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• Vuilleumier S, Chistoserdova L, Lee M-C, Bringel F, Lajus A, Gourion B, Barbe V, Chang J, Cruveillier S, Dossat C, Gillett W, Gruffaz C, Haugen E, Hourcade E, Levy R, Mangenot S, Muller E, Nadalig T, Pagni M, Penny C, Peyraud R, Robinson RG, Roche D, Rouy Z, Saenampechek C, Salvignol G, Vallenet D, Wu Z, Zhou Y, Marx JC, Vorholt JA, Olson MV, Kaul R, Weissenbach J, Médigue C, Lidstrom ME (2009) Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources. Plos One 4:e5584.
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• Peyraud R, Kiefer P, Christen P, Massou S, Portais JC, Vorholt JA (2009) Determination of the ethylmalonyl-CoA pathway using ¹³C metabolomics. Proc. Natl. Acad. Sci USA 106:4846-4851.
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• Francez-Charlot A, Frunzke J, Reichen C, Zingg Ebneter J, Gourion B, Vorholt JA (2009) Sigma factor mimicry involved in regulation of general stress response. Proc. Natl. Acad. Sci. USA 106:3467-3472.
  (This article has been selected as "Research Highlight" in Nature Reviews Microbiology, April 2009)
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• Schrader J, Schilling M, Holtmann D, Sell D, Villela Filho M, Marx A, Vorholt JA (2009) Methanol based industrial biotechnology: current status and future perspectives of methylotrophic bacteria. Trends Biotechnol. 27:106-115. (Review)
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2008

• Kiefer P, Portais JC, Vorholt JA (2008) Quantitative metabolome analysis using liquid chromatography high resolution mass spectrometry. Anal. Biochem. 382:94-100.
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• Spirig T, Tiaden A, Kiefer P, Buchrieser C, Vorholt JA, Hilbi H (2008) The Legionella autoinducer synthase LqsA produces an alpha-hydroxyketone signaling molecule. J. Biol. Chem. 283:18113-18123.
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• Knief C, Frances L, Cantet F, Vorholt JA (2008) Cultivation-independent characterization of Methylobacterium populations in the plant phyllosphere by automated ribosomal intergenic spacer analysis (ARISA). Appl. Environ. Microbiol. 74:2218-2228. 
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• Gourion B, Francez-Charlot, A, Vorholt JA (2008) PhyR is involved in the general stress response of Methylobacterium extorquens AM1. J. Bacteriol. 90:1027-1035.
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2007

• Chistoserdova L, Crowther GJ, Vorholt JA, Skovran E, Portais JC, Lidstrom ME (2007) Identification of a fourth formate dehydrogenase in Methylobacterium extorquens AM1 and confirmation of the essential role of formate oxidation in methylotrophy. J. Bacteriol. 189: 9076-9081.
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• Delaspre F, Nieto Penalver CG, Saurel O, Kiefer P, Gras E, Milon A, Boucher C, Genin S, Vorholt JA (2007) The Ralstonia solanacearum pathogenicity regulator HrpB induces 3-hydroxy-oxindole synthesis. Proc. Natl. Acad. Sci USA, 104:15870-15875.
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2006

• Penalver CG, Cantet F, Morin D. Haras D, Vorholt JA (2006) A plasmid-encoded truncated luxI homolog controls quorum sensing systems and extracellular carbohydrate production in Methylobacterium extorquens AM1. J. Bacteriol., 188:7321-7324.
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• Gourion B., Rossignol M., and Vorholt J.A. (2006) A proteomic study of Methylobacterium extorquens reveals a novel type of response regulator essential for epiphytic growth. Proc. Natl. Acad. Sci USA. 103, 13186-13191.
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• Nieto Penalver C.G., Morin D., Cantet F., Saurel O., Milon A.,  and Vorholt J.A. (2006) Methylobacterium extorquens AM1 produces a novel type of acyl-homoserine lactone with a double unsaturated side chain under methylotrophic growth conditions. FEBS Lett. Jan 23;580(2):561-7.
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2005

• Chistoserdova, L., Vorholt, J.A. & Lidstrom, M.E. (2005) A genomic view of methane oxidation by aerobic bacteria and anaerobic archaea. Genome Biol. 6:208.
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• Sy, A., Timmers, A.C., Knief, C. & Vorholt, J.A. (2005)  Methylotrophic metabolism is advantageous for Methylobacterium extorquens during colonization of Medicago truncatula under competitive conditions. Appl. Environ. Microbiol. 71, 7245-7252.
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• Vorholt, J.A., Kalyuzhnaya, M.G., Hagemeier, C.H., Lidstrom, M.E. & Chistoserdova, L. (2005)  MtdC, a novel class of methylene tetrahydromethanopterin dehydrogenases. J. Bacteriol. 187, 6069-6074.
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• Neculai, A.M., Neculai, D., Griesinger, C., Vorholt, J.A. & Becker, S. (2005)  A dynamic zinc redox switch. J. Biol. Chem. 280:2826-2830.
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• Acharya, P., Goenrich, M., Hagemeier, C.H., Demmer, U., Vorholt, J.A., Thauer R.K. & Ermler, U. (2005)  How an enzyme binds the C1 carrier tetrahydromethanopterin. Structure of the tetrahydromethanopterin-dependent formaldehyde-activating enzyme (Fae) from Methylobacterium extorquens AM1. J. Biol. Chem. 280, 13712-13719.
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2004

• Laukel, M., Rossignol, M., Borderies, G., Völker, U. & Vorholt, J.A. (2004) Comparison of the proteome of Methylobacterium extorquens AM1 grown under methylotrophic and nonmethylotrophic conditions. Proteomics 4:1247-1264
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• Chistoserdova, L., Jenkins, C., Kalyuzhnaya, M.G., Marx, C.J., Lapidus, A., Vorholt, J.A., Staley, J.T. & Lidstrom, M.E. (2004)  The enigmatic planctomycetes may hold a key to the origins of methanogenesis and methylotrophy. Mol. Biol. Evol. 21:1234-1241.
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• Chistoserdova, L., Laukel, M., Portais, J.-C., Vorholt, J.A. & Lidstrom, M.E. (2004)  Multiple formate dehydrogenase enzymes in the facultative methylotroph Methylobacterium extorquens AM1 are dispensable for growth on methanol. J. Bacteriol. 186:22-28.
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2003

• Laukel, M., Chistoserdova, L., Lidstrom, M.E. and Vorholt, J.A. (2003)  The tungsten-containing formate dehydrogenase from Methylobacterium extorquens AM1: purification and properties. Eur. J. Biochem. 270:325-333.
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• Marx C.J., Laukel, M., Vorholt, J.A., and Lidstrom, M.E. (2003) Purification of the formate-tetrahydrofolate ligase from Methylobacterium extorquens AM1 and demonstration of its requirement for methylotrophic growth. J Bacteriol. 185:7169-75.
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2002

• Vorholt, J.A. (2002)  Cofactor-dependent pathways of formaldehyde oxidation in methylotrophic bacteria. Arch. Microbiol. 178:239-249.
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• Ermler, U., Hagemeier, C., Roth, A., Demmer, U., Grabarse, W., Warkentin, E. and Vorholt, J.A. (2002)  Structure of methylene-tetrahydromethanopterin dehydrogenase from Methylobacterium extorquens AM1. Structure 10:1127-1137.
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• Pomper, B.K., Saurel, O., Milon, A. and Vorholt, J.A. (2002)  Generation of formate by the formyltransferase/hydrolase complex (Fhc) from Methylobacterium extorquens AM1. FEBS Lett. 523:133-137.
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• Vorholt, J.A. and Thauer, R.K. (2002)  Molybdenum and tungsten enzymes in C1 metabolism. In : Molybdenum and Tungsten. Their Roles in Biological Processes, Vol 39 'Metal Ions in Biological Systems', A. Sigel and H. Sigel (eds) M. Dekker, Inc ; New York, pp. 571-619.
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• Goenrich, M., Bartoschek, S., Hagemeier, C.H., Griesinger, C. and Vorholt, J.A. (2002)  A glutathione-dependent formaldehyde-activating enzyme (Gfa) from Paracoccus denitrificans detected and purified via two-dimensional proton exchange NMR spectroscopy. J. Biol. Chem. 277:3069-3072.
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• Goenrich, M., Bursy, J., Hübner, E., Linder, D., Schwartz, A.C. and Vorholt, J.A. (2002)  Purification and characterization of the methylene tetrahydromethanopterin dehydrogenase MtdB and the methylene tetrahydrofolate dehydrogenase FolD from Hyphomicrobium zavarzinii ZV580. Arch Microbiol. 177:299-303.
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2001-1995

• Pomper, B.K. and Vorholt, J.A. (2001)  Characterization of the formyltransferase from Methylobacterium extorquens AM1. Eur. J. Biochem. 268:4769-4775.
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• Hagemeier, C.H., Bartoschek, S., Griesinger, C., Thauer, R.K. and Vorholt, J.A. (2001) Re-face stereospecificity of NADP dependent methylenetetrahydromethanopterin dehydrogenase from Methylobacterium extorquens AM1 as determined by NMR spectroscopy. FEBS Lett. 494:95-98.
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• Vorholt, J.A., Marx, C.J., Lidstrom, M.E., Thauer, R.K. (2000)  Novel formaldehyde-activating enzyme in Methylobacterium extorquens AM1 required for growth on methanol. J. Bacteriol. 182, 6645-6650.
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• Hagemeier, C. H., Chistoserdova, L., Lidstrom, M. E., Thauer, R. K., Vorholt, J. A. (2000)  Characterization of a second methylene tetrahydromethanopterin dehydrogenase from Methylobacterium extorquens AM1. Eur. J. Biochem. 267, 3762-3769.
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• Chistoserdova, L., Gomelsky, L., Vorholt, J. A., Gomelsky, M., Tsygankov, Y. D., Lidstrom, M. E. (2000)  Analysis of two formaldehyde oxidation pathways in Methylobacillus flagellatum KT, a ribulose monophosphate cycle methylotroph. Microbiology 146, 233-238.
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• Bartoschek, S., Vorholt, J. A., Thauer, R. K., Geierstange, B. H., Griesinger, C. (2000)  N-Carboxymethanofuran (carbamat) formation from methanofuran and CO2 in methanogenic archaea: thermodynamics and kinetics of the spontaneous reaction. Eur. J. Biochem. 267, 3130-3138.
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• Vorholt, J. A., Chistoserdova, L., Stolyar, S. M., Thauer, R. K. & Lidstrom, M. E. (1999)  Distribution of tetrahydromethanopterin-dependent enzymes in methylotrophic bacteria and phylogeny of methenyl tetrahydromethanopterin cyclohydrolases. J. Bacteriol. 181, 5750-5757.
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• Pomper, B. K., Vorholt, J. A., Chistoserdova, L., Lidstrom, M. E. & Thauer, R. K. (1999)  A methenyl tetrahydromethanopterin cyclohydrolase and a methenyl tetrahydrofolate cyclohydrolase in Methylobacterium extorquens AM1. Eur. J. Biochem. 261, 475-480.
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• Hans, M., Sievers, J., Müller, U, Bill, E., Vorholt, J. A., Linder, D. & Buckel, W. (1999)  2-Hydroxyglutaryl-CoA dehydratase from Clostidium symbiosum. Eur. J. Biochem. 265, 404-414.
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• Grabarse, W., Vaupel, M., Vorholt, J. A., Shima, S., Thauer, R. K., Wittershagen, A., Bourenkov, G., Bartunik, H. D. & Ermler, U. (1999)  The crystal structure of methenyltetrahydromethanopterin cyclohydrolase from the hyperthermophilic archaeon Methanopyrus kandleri. Structure 7, 1257-1268.
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• Vorholt, J. A., Chistoserdova, L., Lidstrom, M. E. & Thauer, R. K. (1998)  The NADP-dependent methylene tetrahydromethanopterin dehydrogense in Methylobacterium extorquens AM1. J. Bacteriol. 180, 5351-5356.
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• Chistoserdova, L., Vorholt, J. A., Thauer, R. K. & Lidstrom, M. E. (1998)  C1 transfer enzymes and coenzymes linking methylotrophic bacteria and methanogenic archaea. Science 281, 99-102.
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• Künkel, A., Vorholt, J. A., Thauer, R. K. & Hedderich, R. (1997) An E. coli hydrogenase 3 type hydrogenase in methanogenic archaea. Eur. J. Biochem. 252, 467-476.
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• Vorholt, J. A. & Thauer, R. K. (1997)  The active species of "CO2" utilized by formylmethanofuran dehydrogenase from methanogenic Archaea. Eur. J. Biochem. 248, 919-924.
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• Vorholt, J. A., Hafenbradl, D., Stetter, K. O. & Thauer, R. K. (1997)  Pathways of autotrophic CO2 fixation and of dissimilatory nitrate reduction to N2O in Ferroglobus placidus. Arch. Microbiol. 167, 19-23.
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• Vorholt, J. A., Vaupel., M. & Thauer, R. K. (1997)  A selenium-dependent and a selenium-independent formylmethanofuran dehydrogenase and their transcriptional regulation in the hyperthermophilic Methanopyrus kandleri. Mol. Microbiol. 23, 1033-1042.
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• Kunow, J., Shima S., Vorholt, J. A. & Thauer, R. K. (1996)  Primary structure and properties of the formyltransferase from the mesophilic Methanosarcina barkeri: comparison with the enzyme from thermophilic and hyperthermophilic methanogens. Arch. Microbiol. 165, 97-105.
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• Vorholt, J. A., Vaupel, M. & Thauer, R. K. (1996)  A polyferredoxin with eight [4Fe-4S] clusters as a subunit of molybdenum formylmethanofuran dehydrogenase from Methanosarcina barkeri. Eur. J. Biochem. 236, 309-317.
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• Vorholt, J. A., Kunow, J., Stetter, K. O. & Thauer, R. K. (1995)  Enzymes and coenzymes of the carbon monoxide dehydrogenase pathway for autotrophic CO2 fixation in Archaeoglobus lithotrophicus and the lack of carbon monoxide dehydrogenase in the heterotrophic A. profundus. Arch. Microbiol. 163, 112-118.